Anti-β-Actin (BA3R)

EBI's Anti-β-Actin (BA3R) Loading Control mAb recognizes both the native and denatured forms of β-Actin from the following species: human, mouse, rat, rabbit, and chicken. β-Actin from other species may also be detectable.

Data Sheet

Print Version	Anti-β-Actin PDF Data Sheet (requires PDF Reader)
Description	Anti-β-Actin (BA3R) Loading Control Monoclonal Antibody
Clonality	BA3R
Host Species	Mouse
Isotype	IgG2b
Immunogen	β-Actin N-terminal peptide-KLH conjugates.
Specificity	Recognizes native and denatured forms of β-Actin (∼42kDa).
Cross Reactivity	β-Actin from human, mouse, rat, rabbit, and chicken. β-Actin from other species may also be detectable.
Purification	Protein A affinity chromatography from mouse ascites fluid.
Applications	Dot, ELISA, IS, WB (see notations).
Form	Liquid: 1mg/mL in 10mM PBS, pH 7.2, 0.05% NaN3.. Lyophilized: 1mg/mL lyophilized in 10mM PBS, pH 7.2, with 1% BSA and 0.05% NaN3.
Working Conditions	WB (with ECL): 1:1000-5,000 dilution (incubate for one hour at room temperature). For best results with other assays (e.g.: Dot, ELISA, IS, etc), please determine optimal working dilution by titration test.
Storage	Liquid: Store at -20°C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks. Lyophilized: Store at -20°C for 3 years or more. Reconstitute with distilled water to desired concentration before use. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks. See Storage for more information.
Images	Fig. 01 EBI's three loading control mAbs reacting against 10μg/lane of mouse brain tissue lysates. 50kDa band is Anti-β-Tubulin (BT7R) at 1:2000 dilution (0.5μg/mL); 42kDa band is Anti-β-Actin (BA3R) at 1:1000 dilution (1μg/mL); 37kDa band is Anti-GAPDH (GA1R) at 1:5000 dilution (0.2μg/mL). Fig. 1:1000 (1μg/mL) Ab dilution used in WB of 20μg/lane tissue lysates from human (1), mouse (2), rat (3), and rabbit (4).