Anti-ERK1 (E19)

EBI's Anti-ERK1 (E19) Signal Transduction mAb recognizes only the ERK1 protein, but not the ERK2 protein. This mAb (similar to E32) has particularly high affinity, and therefore is very effective for immunoprecipitation applications.

Data Sheet

Print Version	Anti-ERK1 PDF Data Sheet (requires PDF Reader)
Description	Anti-ERK1 (E19) Signal Transduction Monoclonal Antibody
Clonality	E19
Host Species	Mouse
Isotype	IgG1
Immunogen	Recombinant full length ERK1 protein.
Specificity	Recognizes only the ERK1 protein.
Cross Reactivity	WB ERK1 from human and mouse cells. IP ERK1 from human cells. ERK1 from other species may also be detectable.
Purification	Protein A affinity chromatography from mouse ascites fluid.
Applications	IP, WB (see notations).
Form	Liquid: 1mg/mL in 10mM PBS, pH 7.2, 0.05% NaN3. Lyophilized: 1mg/mL lyophilized in 10mM PBS, pH 7.2, with 1% BSA and 0.05% NaN3.
Working Conditions	WB (with ECL): 1:1000-10,000 dilution (incubate for one hour at room temperature). For best results with other assays (e.g.: IP, etc), please determine optimal working dilution by titration test.
Storage	Liquid: Store at -20°C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks. Lyophilized: Store at -20°C for 3 years or more. Reconstitute with distilled water to desired concentration before use. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks. See Storage for more information.
Images	Fig. LEFT: 1:10,000 (0.1μg/mL) Ab dilution used in WB of HEK293 cell lysate; 1μg (1), 5μg (2), and 10μg (3) of cell lysate used; RIGHT: IP of anti-ERK1 using 50μg of Jurkat cell lysate; 0.5μg (1), 1μg (2), and 2μg (3) of anti-ERK1 used.