> Anti-ERK1/2 (E31R)
EBI's Anti-ERK1/2 (E31R) Signal Transduction mAb recognizes both the ERK1 and ERK2 proteins from human cells. ERK1 and ERK2 proteins from other species may also be detectable.
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| Print Version | Anti-ERK1/2 PDF Data Sheet (requires PDF Reader) |
|---|---|
| Description | Anti-ERK1/2 (E31R) Signal Transduction Monoclonal Antibody |
| Clonality | E31R |
| Host Species | Mouse |
| Isotype | IgG1 |
| Immunogen | Full length recombinant human ERK1 protein. |
| Specificity | Recognizes human ERK1 and ERK2 proteins. |
| Cross Reactivity | WB: ERK1 and ERK2 from human cells. ERK1 and ERK2 from other species may also be detectable. |
| Purification | Protein A affinity chromatography from mouse ascites fluid. |
| Applications | WB (see notations). |
| Form | Liquid: 1mg/mL in 10mM PBS, pH 7.2, 0.05% NaN3. Lyophilized: 1mg/mL lyophilized in 10mM PBS, pH 7.2, with 1% BSA and 0.05% NaN3. |
| Working Conditions | WB (with ECL): 1:1000 dilution (incubate for one hour at room temperature). For best results with other assays, please determine optimal working dilution by titration test. |
| Storage | Liquid: Store at -20°C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks. Lyophilized: Store at -20°C for 3 years or more. Reconstitute with distilled water to desired concentration before use. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks. See Storage for more information. |
| Images |  Fig. 1:1000 (1μg/mL) Ab dilution used in WB of 10μg (1) and 30μg (2) HeLa cell lysates. |