Anti-His Tag (HIS.H8)

EBI's Anti-His Tag (HIS.H8) mAb is one of the most popular anti-His tag Ab on the global market. We estimate that thousands of labs around the world use it. Numerous successful papers published in top scientific journals serve to showcase the high sensitivity and high affinity of this mAb (see Literatures).

Data Sheet

Print Version	Anti-His Tag PDF Data Sheet (requires PDF Reader)
Description	Anti-His Tag (HIS.H8) Monoclonal Antibody
Clonality	HIS.H8
Host Species	Mouse
Isotype	IgG2b
Immunogen	HHHHHH (6x His) synthetic peptide conjugated to KLH.
Specificity	Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags).
Purification	Protein A affinity chromatography from mouse ascites fluid.
Applications	Dot, ELISA, IP, IS, WB (see notations).
Sensitivity	WB (with ECL): 1-2ng of His-tagged fusion proteins.
Form	Liquid: 1mg/mL in 10mM PBS, pH 7.2, 0.05% NaN3. Lyophilized: 1mg/mL lyophilized in 10mM PBS, pH 7.2, with 1% BSA and 0.05% NaN3.
Working Conditions	WB (with ECL): 1:1,000-3,000 dilution (incubate for one hour at room temperature). IS: 1:500-2000 dilution. For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test.
Storage	Liquid: Store at -20°C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks. Lyophilized: Store at -20°C for 3 years or more. Reconstitute with distilled water to desired concentration before use. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks. See Storage for more information.
Images	Fig. 01 LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue). Fig. 02 Comparison between anti-His tag (HIS.H8) mAb with 2 different vendor Abs, probed against a standard ladder (Qiagen Cat. No. 34705) containing five different His-tagged proteins. All Ab dilutions are 1:2,000 (0.5μg/mL). Fig. 03 Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2).