> Anti-His Tag (HIS.H8)
EBI's Anti-His Tag (HIS.H8) mAb is one of the most popular anti-His tag Ab on the global market. We estimate that thousands of labs around the world use it. Numerous successful papers published in top scientific journals serve to showcase the high sensitivity and high affinity of this mAb (see Literatures).

Data Sheet

Print Version Anti-His Tag PDF Data Sheet (requires PDF Reader)
Description Anti-His Tag (HIS.H8) Monoclonal Antibody
Clonality HIS.H8
Host Species Mouse
Isotype IgG2b
Immunogen HHHHHH (6x His) synthetic peptide conjugated to KLH.
Specificity Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags).
Purification Protein A affinity chromatography from mouse ascites fluid.
Applications Dot, ELISA, IP, IS, WB (see notations).
Sensitivity WB (with ECL): 1-2ng of His-tagged fusion proteins.
Form Liquid: 1mg/mL in 10mM PBS, pH 7.2, 0.05% NaN3.
Lyophilized: 1mg/mL lyophilized in 10mM PBS, pH 7.2, with 1% BSA and 0.05% NaN3.
Working Conditions WB (with ECL): 1:1,000-3,000 dilution (incubate for one hour at room temperature).
IS: 1:500-2000 dilution.
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test.
Storage Liquid: Store at -20°C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks.
Lyophilized: Store at -20°C for 3 years or more. Reconstitute with distilled water to desired concentration before use. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks.
See Storage for more information.
Images Anti-His IF
Fig. 01 LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue).

Anti-His WB 01
Fig. 02 Comparison between anti-His tag (HIS.H8) mAb with 2 different vendor Abs, probed against a standard ladder (Qiagen Cat. No. 34705) containing five different His-tagged proteins. All Ab dilutions are 1:2,000 (0.5μg/mL).

Anti-His WB 02
Fig. 03 Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2).