Anti-RFP Tag (RF5R)

EBI's Anti-RFP Tag (RF5R) mAb has high affinity and is very versatile: it recognizes both the native and denatured forms of RFP (Red Fluorescent Protein) and its many variants: tag-RFP, turbo-RFP, DeRed, mCherry, mOrange, etc.

Data Sheet

Print Version	Anti-RFP Tag PDF Data Sheet (requires PDF Reader)
Description	Anti-RFP (Red Fluorescent Protein) Tag (RF5R) Monoclonal Antibody
Clonality	RF5R
Host Species	Mouse
Isotype	IgG1
Immunogen	RFP from the Discosoma sp. (sea anemone) N-terminal peptide-KLH conjugates.
Specificity	Recognizes native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, etc.
Purification	Protein A affinity chromatography from mouse ascites fluid.
Applications	Dot, ELISA, IP, IS, WB (see notations).
Sensitivity	WB (with ECL): 1-10ng of purified RFP or RFP fusion proteins.
Form	Liquid: 1mg/mL in 10mM PBS, pH 7.2, 0.05% NaN3. Lyophilized: 1mg/mL lyophilized in 10mM PBS, pH 7.2, with 1% BSA and 0.05% NaN3.
Working Conditions	WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature). IS: 1:500-2000 dilution. For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test.
Storage	Liquid: Store at -20°C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks. Lyophilized: Store at -20°C for 3 years or more. Reconstitute with distilled water to desired concentration before use. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks. See Storage for more information.
Images	Fig. 1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with RFP-tagged protein vector; untransfected control (1), transfected with TurboRFP (2), and transfected with dsRed (3).