> Anti-V5 Tag (V5.E10)
EBI's Anti-V5 Tag (V5.E10) mAb recognizes V5-tagged fusion proteins.
T004
| Print Version | Anti-V5 Tag PDF Data Sheet (requires PDF Reader) |
|---|---|
| Description | Anti-V5 Tag (V5.E10) Monoclonal Antibody |
| Clonality | V5.E10 |
| Host Species | Mouse |
| Isotype | IgG1 |
| Immunogen | GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH. |
| Specificity | Recognizes V5-tagged fusion proteins. |
| Purification | Protein A affinity chromatography from culture supernatant of hybridoma cells grown in a bioreactor. |
| Applications | Dot, ELISA, IP, IS, WB (see notations). |
| Sensitivity | WB (with ECL): 1-2ng of V5-tagged fusion proteins. |
| Form | Liquid: 1mg/mL in 10mM PBS, pH 7.2, 0.05% NaN3. Lyophilized: 1mg/mL lyophilized in 10mM PBS, pH 7.2, with 1% BSA and 0.05% NaN3. |
| Working Conditions | WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature). IS: 1:500-2000 dilution. For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test. |
| Storage | Liquid: Store at -20°C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks. Lyophilized: Store at -20°C for 3 years or more. Reconstitute with distilled water to desired concentration before use. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks. See Storage for more information. |
| Images |  Fig. 1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (1) and transfected (2). |